| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_1e96496a9949403e2af2ca913e65bfec |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P21-02 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P21-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q3-00 |
| classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-02 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q3-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-00 |
| filingDate |
2015-03-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
2018-04-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c3d80f471dca8c3e440d63e580fdef61
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fbcd0054312d81f2e6637163234d2774
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_554a66601be8659f8329e94403ca7bae
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_319870e838688c1647db982b8cecf771 |
| publicationDate |
2018-04-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-9951392-B2 |
| titleOfInvention |
Substrate replenishment and byproduct removal improve yeast cell-free protein synthesis |
| abstract |
Methods and kits are provided for calibrating a cell-free protein synthesis reaction for optimal activity. The method includes the steps of providing an extract competent for cell-free protein synthesis (CFPS); performing cell-free protein synthesis with the extract; measuring a first reaction end-point where in vitro protein synthesis plateaus; measuring a second reaction end-point where Energy Charge of the extract declines to a level in a range from about 0.40 to about 0.80 of Energy Charge of a control extract; and adjusting Energy Charge of the extract to a level in a range from about 0.80 to about 1.0 of the control extract. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11542538-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11725224-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11673921-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11530432-B2 |
| priorityDate |
2014-03-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |