| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_6b4a80ac02f1ecd0e698ec665e9b36b6 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-22
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P21-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-63
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-755 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-755
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-22 |
| filingDate |
2016-08-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
2018-03-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3280d369a98b74c90be0fc79ab7ceb9e
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2b8fa21d5a985e995b7dcf6e82bc113f
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_df2a46b9a93ab80d681597cfa31eb3b4
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_935170919994c40a6293ff3b6b1247e9
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7835958f416dd0286b507bd3858c53d9
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_924cf51acf7986f93393a37d8e09be3f |
| publicationDate |
2018-03-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-9926361-B2 |
| titleOfInvention |
Process for producing and purifying factor VIII and its derivatives |
| abstract |
Disclosed is a method for producing proteins having factor VIII procoagulant activity in serum-free medium by in vitro culturing of mammalian cells, wherein the serum-free medium contains an inhibitor against the protease released from cultured cells. In accordance with this invention, the inhibitor can protect the cleavage of a target protein during cultivation and increase homogeneity of a target molecule, wherein the inhibitor can be a dextran sulfate. This invention also relates to a method of purifying target molecules from the culture medium containing both a target molecule and selected inhibitors by affinity chromatography. |
| priorityDate |
2007-02-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |