| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_8d527006cf21db3688c9b1c861cf8c44 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2525-117
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2523-125
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2535-122
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2525-191 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1093
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C40B40-06
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C40B40-08
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C40B50-06
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6806
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6869
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6876 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C40B50-06
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C40B40-06
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C40B40-08 |
| filingDate |
2012-11-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
2018-03-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6dcb1a11f83e4617349eaf9810901d0e
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_817fe156c9567e16d9bdaa77f4cf53ef
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b5a940ce947ecc424e42b075fe951831
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6f67923a7f51816e2c9eb14e6c3fdfba
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_752cf087e4a5fc300e5788bda715379e
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b3e2d7d0f70bf29c79c6eb068d4a34a4 |
| publicationDate |
2018-03-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-9920363-B2 |
| titleOfInvention |
Constructing method of high-throughput sequencing library and use thereof |
| abstract |
The present invention provides a method for constructing a high-throughput sequencing library, which comprises: fragmenting genomic DNA; end-repairing the DNA fragments; adding a base A to the 3′ end of the end-repaired DNA fragments; connecting the DNA fragments having cohesive end A with a methylated adapter; carrying out hybrid capture on the connection products by using specific probes to obtain object fragments; treating the object fragments with bisulfite, to convert non-methylated cytosines to uracils; PCR amplifying the converted object fragments; and separating and purifying the amplification products, wherein the amplification products constitute the high-throughput sequencing library. The present invention also provides a method and an apparatus for identifying methylation information in specified genome regions of a sample. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11725251-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11685958-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11410750-B2 |
| priorityDate |
2011-11-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |