Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_17e7b0b4d0b9daafc57d063ea38ac3f4 |
classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-924 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y302-01031 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-40 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-2402 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P17-10 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P17-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-40 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-24 |
filingDate |
2016-03-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate |
2018-03-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e683e0d0cb7d18fec71d90d2437a49e3 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fbb5c044365bb1ae12abd52badb88788 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4691b4851cb1510b4869beba549e6041 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7ea995c606f6a5ce5249aac2cf6fb852 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5a9d7e06c3da939b652fea4ff4490e53 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9d11dd621656c18238c9de8b9670d688 |
publicationDate |
2018-03-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
US-9909111-B2 |
titleOfInvention |
Mutant lactobacillus beta-glucuronidase enzymes with enhanced enzymatic activity |
abstract |
Mutated Lactobacillus brevis strain 269Y β-glucuronidase enzymes with enhanced enzymatic activity at low pH (e.g., below pH 6.8), as well as enhanced thermostability as compared to wild type enzyme are provided. The enzymes of the invention advantageously allow for accurate analysis of bodily samples for the presence of drugs at low pH and in 30 minutes or less, as compared to the several hours needed using prior enzyme preparations. Methods of using the mutated enzymes for hydrolysis of glucuronide substrates, including opiates and benzodiazepines, are also provided. |
isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2022090037-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11268079-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11421210-B2 |
priorityDate |
2016-03-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |