| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_74e1c7f3a6608121e120e6cc430a7986
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_7c2abd09a6e8c8d6bab441582746deba
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_7766064ebf0f1e9534be7d6a239867fd
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_d03a23980e558bd5501fb56d07060b56
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_0c37ea6c0ccf659261758e2450ac848e |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-14 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0662
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0663
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0606
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0607 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-074
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-0775
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-0735 |
| filingDate |
2012-05-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
2018-02-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f54ea4369b8b6bb21f5db631e1bd8572
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_839104c9881525aba321a372817bd01f
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_840083467756dd6076166ed843e738ac
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4d0ab56d320bce7c9b0a85c83800f85d |
| publicationDate |
2018-02-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-9885019-B2 |
| titleOfInvention |
Passaging and harvesting formulation and method for human pluripotent stem cells |
| abstract |
Formulations and methods are disclosed for the harvesting and subsequent passaging of human pluripotent stem cells without the use of enzymes and/or scraping to dislodge cells from cell culture vessels. The formulations and methods permit the harvesting of cells as large clusters from the surface of various cell culture vessels including multilayer cell culture vessels. Further, the formulations and methods provide high yields of harvested cells for subsequent passaging and high post-harvest cell viability. Pluripotent stem cells passaged with the formulations according to the methods remain undifferentiated and express typical stem cell markers, while, at the same time, they retain the differentiation capability and are able to differentiate into the cells in all three germ layers and generate teratomas, even after numerous rounds of harvesting and passaging. These hPSCs also maintain normal karyo-type after passaged with the formulations for extended period of time. |
| priorityDate |
2011-05-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |