http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9879073-B2
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_db304b0b56a0b1201d8754af806cd838 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2317-22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2317-569 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-18 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K16-18 |
| filingDate | 2015-04-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2018-01-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_19af68b9bc0175ff50615293097a4638 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a5ae9e157025507fa35e7ec1109c6472 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_eec7b6da980bd1fbfcc084cc7bd98475 |
| publicationDate | 2018-01-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | US-9879073-B2 |
| titleOfInvention | Lung-targeting nanobodies against human pulmonary surfactant protein A and a method for producing the same |
| abstract | The present invention relates to the field of biochemistry and pharmaceutical technologies. The present invention provides nanobodies that bind to human pulmonary surfactant protein A (SP-A) as well as the preparing methods and use of the same. The nanobody comprise an amino acid sequence having the formula of Q(x) 2 LVESGG(x) 2 V(x) 2 G(x)SL(x)LS(x) 24 E(x) n2 KG(x) 4 S(x) n3 T(x) 2 Y(x)C(x) n4 S(x) n5 V(x) n6 R; wherein x is amino acid; n2˜n6 are positive integers; 1≦n2≦21; 1≦n3≦19; 1≦n4≦50; 1≦n5≦22; 1≦n6≦8. The present invention take fresh frozen sections of lung as antigen, gene sequences with high affinity with hSP-A were obtained by constructing an SP-A antibody library and affinity selection, and nanobodies with high affinity and small molecular weight were obtained by induced expression of the gene sequences through a prokaryotic expression vector. Immunohistochemistry and in vivo imaging in nude mice showed the nanobodies have high specificity for targeting lung tissue. |
| priorityDate | 2015-02-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 94.