| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_2d46f2c715f9d4ce6871393d8bbc1b29
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_cd786130acb8ab76dd5009c23d2ca761
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_614bcf80563ca027dab41cc08efa4868
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_e5a9e35c69b65999dd383183e77faec3
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_72ae4306f054183c3dfa448d444be96a
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_6ae2c7cb1e6f8edf1d3dfe246d8ad21b
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_59f27f009afc3740b820198e5d512358 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2317-24
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2317-14 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-18
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-2809
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-815
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-81
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-2896 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-81
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-74
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K16-28
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-04
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K16-18
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K16-00 |
| filingDate |
2011-03-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
2018-01-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3588b4c9d2fffd8281f7c1818c7bd8df
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ee196f2c9630904b2bfa3751fbac8096
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6b403e065ab898c45654cb2ee176a722
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b6e5f0c8e8f6c10a312255d140b64a4d
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_926d7ba0b78a0ac99ff7bad6f7bd9a1c |
| publicationDate |
2018-01-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-9873746-B2 |
| titleOfInvention |
Methods of synthesizing heteromultimeric polypeptides in yeast using a haploid mating strategy |
| abstract |
Methods are provided for the synthesis and secretion of recombinant proteins preferably large mammalian proteins or hetero-multimeric proteins at high levels and for prolonged time in polyploid, preferably diploid yeast. These methods use various mating competent yeast, including Pichia . In a preferred embodiment, a first expression vector is transformed into a first haploid cell; and a second expression vector is transformed into a second haploid cell. The transformed haploid cells, each individually synthesizing a non-identical polypeptide, are identified and then genetically crossed or fused. The resulting diploid strains are utilized to produce and secrete fully assembled and biologically functional hetero-multimeric protein. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11225667-B2 |
| priorityDate |
2003-10-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |