http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9856527-B2
Outgoing Links
Predicate | Object |
---|---|
assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_efb35e154fff5d3177ae8520d41046d5 |
classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2545-101 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2537-143 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6851 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6865 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6813 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-34 |
filingDate | 2016-05-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2018-01-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8ccb9d74d277255eecf1f30e1e94e7d5 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_40b73285ecb042e6c2a83dfac136d532 |
publicationDate | 2018-01-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | US-9856527-B2 |
titleOfInvention | Methods for quantitative amplification and detection over a wide dynamic range |
abstract | Disclosed are compositions and methods for making differentiable amplicon species at unequal ratios using a single amplification system in a single vessel. The number of differentiable amplicons and their ratios to one another are chosen to span the required linear dynamic range for the amplification reaction and to accommodate limitations of the measuring system used to determine the amount of amplicon generated. Unequal amounts of distinguishable amplicon species are generated by providing unequal amounts of one or more amplification reaction components (e.g., distinguishable amplification oligomers, natural and unnatural NTP in an NTP mix, or the like). The amount of target nucleic acid present in a test sample is determined using the linear detection range generated from detection of one or more amplicon species having an amount within the dynamic range of detection. |
priorityDate | 2009-07-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 260.