http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9739715-B2

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filingDate 2016-03-02-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2017-08-22-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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publicationDate 2017-08-22-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber US-9739715-B2
titleOfInvention Laser scanning microscope system and method of setting laser-light intensity value
abstract A method of setting a laser-light intensity value includes: emitting laser light, the laser light being excitation light, a fluorescent-dyed biological sample being irradiated with the excitation light and emitting light; detecting fluorescence emitted by the biological sample, and outputting a signal corresponding to a brightness value; prestoring relation information, the relation information including the plurality of laser-light intensity values, and information on at least one possible correlation between a phototoxicity degree and the brightness value in relation to each of the laser-light intensity values, the phototoxicity to the biological sample resulting from the laser light; generating a fluorescence image having the brightness value based on the output signal; calculating a brightness value representative of a ROI area based on the generated fluorescence image; and referring to the relation information, and determining a laser-light intensity value satisfying tolerance of the phototoxicity based on the calculated representative brightness value.
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Total number of triples: 27.