| abstract |
The present invention relates to a disposable device ( 100 ) for amplifying at least one target nucleic acid present in a liquid and biological sample of interest, which consists of a solid body ( 2 ), at least one fluid channel ( 3 ) connecting an inlet ( 4 ), via which all or part of the sample of interest can be drawn up and/or discharged, and an outlet ( 5 ), which is itself connected to a means for the drawing up/discharging of the said sample of interest, the fluid channel ( 3 ) further comprising from the inlet ( 4 ) to the outlet ( 5 ):n a first compartment ( 8 ) containing all or part of the thermostable constituents, a means ( 15 ) for mixing the constituents with the sample of interest, a second compartment ( 9 ) containing all or part of the non-thermostable constituents, and in addition, at least one zone intended for heating the said sample of interest ( 6 ) mixed with the said amplification constituents in order to allow the amplification of the target nucleic acid. nn The invention also proposes an amplification method using such a device. n The said invention has a preferred application in the field of medical diagnosis. |