http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9663769-B2
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_a888a536389af4a49ebdc2c36de65848 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-1247 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y207-07006 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-12 |
| filingDate | 2015-10-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2017-05-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8f873f9fb9e3f7a3650c711026abaf9a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_68b99ca327c9561ffd3e179d14536cb1 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a53e8721577b2fea399d5a779eb4c322 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e3f124a12be78e5f9d000a2b67e1ed4a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f81a18a76363ea85b228c3611ccaa730 |
| publicationDate | 2017-05-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | US-9663769-B2 |
| titleOfInvention | T7 RNA polymerase variants with cysteine-serine substitutions |
| abstract | The present disclosure provide novel variants of T7 RNA polymerase. Embodiments of T7 variants, according to the instant invention, include a Cysteine-Serine substitution on position 723 of the amino acid sequence of the T7 polypeptide. Embodiments of T7 variants according to the instant invention have a DNA-dependent RNA polymerase enzymatic activity and a reduced tendency to form intramolecular homodimers by way of oxidizing thiol groups. The amino acid substitutions within the T7 variants disclosed herein impact minimally, if at all, the RNA polymerase activity of the T7 polypeptide. Further, the mutations of the disclosed embodiments may optionally be combined with mutations which provide enhanced thermostability compared to the wild-type reference. |
| priorityDate | 2011-04-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 143.