Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_30783f5e0439f26fb23d0089d47f0ea6 |
classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-916 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-904 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-91074 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-938 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-33 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2334-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2021-3125 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-31 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-25 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-272 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-00 |
classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-33 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-25 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-31 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-27 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-35 |
filingDate |
2012-09-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate |
2017-04-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e7d01a84fead5db577262235614d64a8 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_04a737446eeedbe21bca9b51df70c687 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_77a8464f8be3838bb2a88bc31a3b4d58 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_245d055ddf9ef6ff33f7123b7a4a05b8 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_63cd9c9a0bc4f085331892671ef69946 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f064169ef24d535f3a202016b6e8079d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fa3237bc184a0f2fcdc3f6b901d303eb |
publicationDate |
2017-04-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
US-9611502-B2 |
titleOfInvention |
Method for simultaneously measuring activity of various enzymes by using multi-wavelength absorption single channel |
abstract |
A method for simultaneously measuring the activities of multiple kinds of enzymes in single channel through concimitantly monitoring multiple wavelength absorbances is claimed; based on linear additivity of absorbances and the isoabsorbance wavelengths of chromogenic substrates and their chromogenic products, both the principles for the combination of chromogenic substrates required for simultaneously measuring the activities of multiple kinds of enzymes in single channel through concimitantly monitoring multiple wavelength absorbances and an approach for data processing to eliminate the interference of the overlapped absorbances are developed, kinetic analysis of reaction curves via numerical integration eliminates the interference of all substrates and products and estimates the maximal reaction rates of the enzymes to be measured, giving the linear ranges and the limits of quantification for simultaneously measuring the activities of multiple kinds of enzymes in single channel comparable to those by separate assays; the present invention is applicable for simultaneously measuring the activities of multiple kinds of enzymes in biological samples, simultaneously measuring multiple components by enzyme-labeled immunoassays, screening simultaneously of inhibitors against multiple targets, for the practice in clinical biochemical analyses, clinical immunoassays, health laboratory analyses, the discovery of inhibitors, and the basical researches which need the present invention. |
isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2021067565-A1 |
priorityDate |
2012-09-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |