http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9606126-B2
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_46360d89a704bba8c618faecccec617e http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_df64f2cb3bebfa7855fa356e0915de4d http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_82557442269d435ec5e9e74e70b8c08f |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01D15-3804 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C30B7-08 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-6803 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/B01D15-38 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C30B7-08 |
| filingDate | 2010-06-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2017-03-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_75c227a34873b4bf8aa772aa778dd7e0 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_dcd0e67a3f26b615cb94585d8ea2f535 |
| publicationDate | 2017-03-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | US-9606126-B2 |
| titleOfInvention | Compositions and methods for membrane protein detergent stability screen |
| abstract | The invention provides methods to assess protein stability and to obtain sizing information. In one aspect, the screen comprises a 94 detergent panel and a series of MWCO filtered microplates. A protein of interest is bound to an affinity matrix and aliquoted into a 96-well microplate. Wells containing the immobilized protein are washed in the new detergent and then eluted in the new detergent into a collection plate. Protein not stable in the new detergent is precipitated on the resin and not present in the elutions. Half of the elution is passed through a high (i.e., 300 kDa) MWCO microplate and the other half through a low (i.e., 100 kDa) MWCO microplate. Elutions from the microplates are spotted on a nitrocellulose membrane, visualized by Western analysis (or by some other method), and quantified. The high MWCO provides stability readout and the ratio of low/high kDa provides sizing information. |
| priorityDate | 2009-06-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 612.