| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_50eb752940fa4aa36756a6bc9f6f3504 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-64
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-66
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1031
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-10 |
| classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-14 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-66
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-64
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-34
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 |
| filingDate |
2015-10-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
2017-02-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_09d3eda9364e7e3f8a070d5df5c1da95
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c22111a37054feb348dd1710615f4a98
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_578f41378abf7ab3f5289619954b9c69
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_df25ebad11b4626dbe8036244088360b
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c6243926a4abf1b19fd3efb8d32de8e2
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c34800753595353e2e6938c1405769a5
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5297213fcdef57cdd287b76ca1bc2780
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3e09464a433b89ea9bf5043193b34ce0
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_973ff7ce33b9c5f730a90445e1a94cfb
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1b0e65f6722dab22c8233aa56f25f2bc |
| publicationDate |
2017-02-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-9580715-B2 |
| titleOfInvention |
Nuclease-mediated DNA assembly |
| abstract |
Methods are provided herein for assembling at least two nucleic acids using a sequence specific nuclease agent (e.g., a gRNA-Cas complex) to create end sequences having complementarity and subsequently assembling the overlapping complementary sequences. The nuclease agent (e.g., a gRNA-Cas complex) can create double strand breaks in dsDNA in order to create overlapping end sequences or can create nicks on each strand to produce complementary overhanging end sequences. Assembly using the method described herein can assemble any nucleic acids having overlapping sequences or can use a joiner oligo to assemble sequences without complementary ends. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10273488-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11111504-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10626402-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9738897-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11499164-B2 |
| priorityDate |
2014-06-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |