http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9498500-B2
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_0931241d9441d023245580a92141bf68 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-734 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-34 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0663 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K35-28 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K35-28 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12M1-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-24 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-0775 |
| filingDate | 2015-04-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2016-11-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b989c3acd1e562fcdfe5e9e42f166ad0 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_93eeee302dc3e2a8e139a1a4fc0d73e0 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_01383e2c15c87f5eaa98af87a3a304e8 |
| publicationDate | 2016-11-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | US-9498500-B2 |
| titleOfInvention | Method of washing adherent cell using trehalose-containing cell-washing solution |
| abstract | Methods of washing adherent cells, capable of effectively suppressing cell death due to proteolytic enzyme treatment for detaching the adherent cell from a culture vessel and subsequent cell treatment; cell-washing solutions used for the washing method; methods of producing cell suspensions for transplantation using the cell-washing solution; and kits comprising the cell-washing solution. Trehalose or its derivative or a salt thereof is added to physiological aqueous solutions to prepare cell-washing solutions containing trehalose or its derivative or a salt thereof as an active ingredient. The cell-washing solutions can be used to wash adherent cells before detaching the adherent cells from a culture vessel by proteolytic enzyme treatment to suppress cell death due to the proteolytic enzyme treatment. The concentration of trehalose applied to the cell-washing solution may be a concentration capable of suppressing the cell death due to the proteolytic enzyme treatment, such as 0.1 to 20 (w/v) %. |
| priorityDate | 2012-09-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 424.