| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_1d28879a9b44cb0ff0cbf6ac946c4b3a |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2537-165
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2565-513
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2565-537
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2533-107
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2565-102
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2565-518
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2565-137 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B82Y15-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B82Y30-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G16B50-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6869
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6874
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6837
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6844
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-68 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/B82Y30-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/B82Y15-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-34
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate |
2013-10-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
2016-11-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a68cf22722b6b1e3d8c648c371baa967
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_31101076208c49e80c8b767be2f74d53
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_12a449f6980529a6b763fbb61354b721
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0c742bb4d77831b833cbbda92ce8525f |
| publicationDate |
2016-11-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-9493830-B2 |
| titleOfInvention |
Reagents, methods, and libraries for bead-based sequencing |
| abstract |
The present invention provides methods for determining a nucleic acid sequence by performing successive cycles of duplex extension along a single stranded template. The cycles comprise steps of extension, ligation, and, preferably, cleavage. In certain embodiments the methods make use of extension probes containing phosphorothiolate linkages and employ agents appropriate to cleave such linkages. The invention provides methods of determining information about a sequence using at least two distinguishably labeled probe families. In certain embodiments the methods acquire less than 2 bits of information from each of a plurality of nucleotides in the template in each cycle. In certain embodiments the sequencing reactions are performed on templates attached to immobilized beads. The invention further provides sets of labeled extension probes containing phosphorothiolate linkages. In addition, the invention includes performing multiple sequencing reactions on a single template by removing initializing oligonucleotides and extended strands and performing subsequent reactions using different initializing oligonucleotides. |
| priorityDate |
2005-02-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |