| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_e25a8f8a6716fa7d3f4f964dc3a1db39
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_32d08a1aa8a8207ff262ceea8adaa07a
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_debd2d48f1d16ee68e2c460dc572a83f
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_6f6cf36cc0a709cc161d62b92f32bfbb
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_f5f4938993980add0c5cc8139415df2e |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-102 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 |
| filingDate |
2010-12-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
2016-09-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_24fdc7b2ef8f087dcfbe6fdfaa3574b0
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2e03bacdd133df203d6654930b1455ae
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d283ca8c3cefcf16a5b206a0e96e2eb8
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_36272ed213d253a273a3c0d302705649 |
| publicationDate |
2016-09-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-9453216-B2 |
| titleOfInvention |
Mutagenesis method |
| abstract |
The present invention provides a mutagenesis method wherein a nucleic acid molecule is mutagenized with at least one mutagenesis primer in a primer extension reaction and subsequently amplified by rolling circle amplification (RCA). The method involves the step of rendering the template strand unfavorable for RCA. The method involves steps leading to selective amplification of only the mutated strand by a strand-displacing DNA polymerase. Multiple copies of the mutated plasmids are generated during multiple-primed RCA and the resulting DNA is transformed for use. The method is suitable for mutating both single-stranded and double-stranded DNA. The present invention also provides a kit for use in the mutagenesis method. |
| priorityDate |
2009-12-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |