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filingDate 2011-05-08-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2016-09-13-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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publicationDate 2016-09-13-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber US-9442106-B2
titleOfInvention Simple and affordable method for immunophenotyping using a microfluidic chip sample preparation with image cytometry
abstract The system includes a simple system for CD4 and CD8 counting in point-of-care HIV staging within resource poor countries. Unlike previous approaches, no sample preparation is required with the sample added directly to a chip containing dried reagents by capillary flow. A large area image cytometer consisting of an LED module is used to excite the fluorochromes PerCP and APC labeled targets and a monochrome CCD camera with a combination of two macro lenses captures images of 40 mm 2 of blood (approximately 1 micro liter). CD4 and CD8-T-lymphocyte counts correlate well with those obtained by flow cytometry. The cytometer system described in the present invention provides an affordable and easy-to-use technique for use in remote locations.
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