| abstract |
This disclosure provides methods and compositions for amplification and sequencing of DNA templates, comprising at least two of: 2′-deoxyinosine-5′ triphosphate, 5-propynyl-2′-deoxycytidine-5′-triphosphate, and 8-oxo-2′-deoxyguanosine-5′-triphosphate. Incorporation of these promoting nucleotides into amplification and sequencing reactions improves the amplification and sequencing of difficult-to-sequence DNA regions such as a GC rich regions or GT rich regions; repetitive sequences, including dinucleotide, trinucleotide, direct, inverted, Alu, poly A or poly T repeats; and hairpin or other secondary structures. |