| abstract |
An Eptifibatide preparation method with product purity of more than 99.5%, the method comprising: using a solid phase polypeptide synthesis method to prepare eptifibatide resin, conducting acidolysis on the Eptifibatide resin to obtain a crude Eptifibatide linear peptide product, oxidizing to obtain a crude Eptifibatide product, purifying and exchanging salt to obtain an Eptifibatide finished product; the method using the solid phase polypeptide synthesis method to prepare the eptifibatide resin is: using a solid phase coupling synthesis method to sequentially splice a corresponding protective amino acid or a segment in the following sequence onto amino resin, and obtaining the Eptifibatide resin: X—Y-Trp(R 1 )-Pro-Cys(R 2 )-amino resin, wherein R 1 is Boc or H, R 2 is Trt or Acm, X is Mpr(R 2 )-Harg(R 3 ), R 3 is Pbf or H, and Y is Gly-Asp(OtBu). |