Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_3e71293e8c653f99fb08d7739f2f425f http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_56541df79a26070c6c2c585b54af47c5 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_e619defdfb162ea953d66b95d250bdf3 |
classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2521-131 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2525-205 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2521-119 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2563-119 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-58 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-582 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P21-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-5308 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-532 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6804 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-58 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-532 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-53 |
filingDate |
2013-06-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate |
2016-07-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2ff1ffcee2bd8aee724edb0a00c91305 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3a628602a60fb643bb603cd2895e243c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2b22fc333dc535759f9bd70460564188 |
publicationDate |
2016-07-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
US-9388454-B2 |
titleOfInvention |
Method for producing protein-nucleic acid conjugate, and method for detecting target substance |
abstract |
Provided is a method for producing a nucleic acid probe that can detect a target substance with good sensitivity. A method for producing a nucleic acid probe, comprising: a 3′-terminal addition step of adding at least one nucleoside triphosphate derivative having a glutamine (Gln) residue or a lysine (Lys) residue to the 3′-terminal of a nucleic acid using a 3′-terminal addition enzyme which adds a nucleotide to the 3′-terminal of a nucleic acid, and a labeling compound binding step of either binding a labeling compound having a lysine (Lys) residue and containing a labeling moiety to the glutamine (Gln) residue using a transglutaminase (TGase), or binding a labeling compound having a glutamine (Gln) residue and containing a labeling moiety to the lysine (Lys) residue using a transglutaminase (TGase). |
isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2022089639-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11786603-B2 |
priorityDate |
2012-06-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |