| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_2efbe95965e682e179dfd266c9980505
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_4032d4610f4b8faa658596427ae7243a
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_1a036559e133cf9145349f432c9caee3
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_658da3351c95cae75f955712944c5e22 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y02P20-52
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y02E50-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y02E50-17 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y302-01037
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y302-01001
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y302-01003
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y401-01001
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y302-01008
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-242
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P7-06
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-2428
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-2414 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P7-06
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-30
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-34
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-28
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-26 |
| filingDate |
2009-05-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
2016-07-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bc8a0f411a3c9a9c97e6d8d796804fed
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0f7d9be7ce1de6eca3204b72e7c8f9ed
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9f8b02154e101473df82df5373f058c2 |
| publicationDate |
2016-07-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-9388399-B2 |
| titleOfInvention |
Development of strains of the thermotolerant yeast Hansenula polymorpha capable of alcoholic fermentation of starch and xylan by expression of starch and xylan degrading enzymes |
| abstract |
Genes SWA2 and GAM1 from the yeast, Schwanniomyces occidentalis , encoding α-amylase and glucoamylase, respectively, were cloned and expressed in H. polymorpha . The expression was achieved by integration of the SWA2 and GAM1 genes into the chromosome of H. polymorpha under operably linked to a strong constitutive promoter of the H. polymorpha -glyceraldehyde-3-phosphate dehydrogenase gene (HpGAP. Resulting transformants acquired the ability to grow on a minimal medium containing soluble starch as a sole carbon source and can produce Ethanol at high-temperature fermentation from starch up to 10 g/L. A XYN2 gene encoding endoxylanase was obtained from the fungus Trichoderma resee , and a xlnD gene coding for β-xylosidase was obtained from the fungus Aspergillus niger . Co-expression of these genes was also achieved by integration into the H. polymorpha chromosome under control of the HpGAP promoter. The resulting transformants were capable of growth on a minimal medium supplemented with birchwood xylan as a sole carbon source. Successful expression of xylanolytic enzymes resulted in a recipient strain capable of fermentation of birchwood xylan to ethanol at 48° C. Further with co expression of the forgoing genes in a H. polymorpha strain that overexpresses a pyruvate decarboxylase gene further improved ethanol production. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2017218402-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10961549-B2 |
| priorityDate |
2008-05-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |