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filingDate 2015-03-09-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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publicationDate 2016-06-14-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber US-9365891-B2
titleOfInvention Nucleic acid analysis device, method for producing same, and nucleic acid analyzer
abstract Disclosed is a technique for binding microparticles to patterned bonding pads of a metal (e.g., gold) formed on a support. The microparticles each carry a nucleic acid synthetase or DNA probe immobilized thereon for capturing a nucleic acid sample fragment. The technique involves forming, on a support surface, a film having a thickness equivalent to that of the bonding pads; controlling the size of microparticles with respect to the size of bonding pads; and thereby immobilizing microparticles each bearing a single nucleic acid sample fragment to the bonding pads in a one-to-one manner in a grid form. This allows high-density regular alignment and immobilization of many types of nucleic acid fragment samples on a support and enables high-throughput analysis of nucleic acid samples. Typically, immobilization of microparticles at 1-micrometer intervals easily provides a high density of 10 6 nucleic acid fragments per square millimeter.
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