http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9353382-B2
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_8abb210936dc3b56af93e346a2335b7f |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2800-108 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-71 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2800-40 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2710-16622 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2710-16222 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-85 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-005 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-85 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-005 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K38-00 |
| filingDate | 2013-10-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2016-05-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_aeaab599ebb2333cc0b3fab37d49c83b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_40b5298fafcbd24c37d37f455623ae11 |
| publicationDate | 2016-05-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | US-9353382-B2 |
| titleOfInvention | Process, vectors and engineered cell lines for enhanced large-scale transfection |
| abstract | Processes, vectors and engineered cell lines for large-scale transfection and protein production in mammalian cells, especially Chinese Hamster Ovary (CHO) cells are described in which transfection efficiencies are realized through the use of a single vector system, the use of functional oriP sequences in all plasmids, the use of codon-optimized Epstein-Barr virus nuclear antigen-1 (EBNA1) constructs, the use of a fusion protein between a truncated Epstein-Barr virus nuclear antigen-1c (EBNA1c) protein and a herpes simplex virus protein VP16, the use of a 40 kDa fully deacetylated poly(ethylenimine) as a transfection reagent, the use of co-expression of a fibroblast growth factor (FGF) and/or the use of protein kinase B to potentiate heterologous gene expression enhancement by valproic acid (VPA). |
| priorityDate | 2007-03-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 79.