Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_2f484087b14f36903cc11311b584d9ee http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_0b74e363c995f71b92b102bdecffe0de http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_e41651508566f0385833e1ba14a21ffd http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_47756a5c8359cc418bb2548888a687fa http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_7a38faee4abf8df55f7a6896776f319f http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_8f803ab4cd19d4d23e4c776b37b49ae9 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_6f6e545e91e7a9537396918bfed4e5e6 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_ead108bb886453bb3c79378708053dcd http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_ebb88ff81919273b4e22aab941f94c7b http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_0b5b1c8ea1165ea3978dac7528b513c9 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_e18ecf66022eff6d10f88e4e8c72eaa4 |
classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2565-626 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-158 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-178 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2537-16 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2563-143 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2563-159 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6886 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-686 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-6486 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-34 |
filingDate |
2012-03-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate |
2016-04-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5b37811c41345d816c1688bd4eef4846 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ab0571f893d2bda62d5b10306bb3bbb2 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b8b32eaab8585bc450565cbd90c1d108 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5d62bb3297fd6e7ec75ef55965a75913 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8f3b78235625e77e6741b18899e636b3 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_022aab3dcf919fbc4f0fd9fabaa2badb http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e461f84253bdef89f60703cd491bddd7 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_58d07dea48994547c1bdd3b8790d7ab1 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_976b9a4a17f7f00a9a6daa023f54e26f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e6b87b7f01dbf0cb806956970e65d2da |
publicationDate |
2016-04-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
US-9322055-B2 |
titleOfInvention |
System and method for determining copies-per-unit-volume using PCR and flow control of droplets |
abstract |
Methods and systems for quantification of a target nucleic acid in a sample are provided. The method includes forming a plurality of discrete sample portions. Each of the plurality of discrete sample portions comprising a portion of the sample, and a reaction mixture. The method further includes amplifying the plurality of discrete sample portions to form a plurality of discrete processed sample portions. At least one discrete processed sample portion containing nucleic acid amplification reaction products. Fluorescence signals are detected from the at least one of the plurality of discrete processed sample portions to determine a presence of the at least one target nucleic acid. The method also includes determining the respective volumes of the plurality of the plurality of discrete processed sample portions, and estimating the number of copies-per-unit-volume of the at least one target nucleic acid in the sample. Estimating the number of copies-per-unit-volume is based on the number of discrete processed sample portions determined to contain the at least one target nucleic acid therein. |
isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10557174-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11193872-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10156509-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11603560-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2016208342-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2020001290-A1 |
priorityDate |
2011-04-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |