Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_6cc367bc90c1255f6e7df778eb4a694a http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_f47d0b4e8434b52f9ca5f5b9d1750951 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_1e268862df7e7104fbccb8f5a6b682eb |
classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2525-186 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2535-122 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2525-185 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2525-207 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2533-107 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6869 |
classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H21-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H21-02 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
filingDate |
2011-05-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate |
2016-02-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_dafec5047513066d70e2b7156891e886 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_947fed29b41f624978862221d03008bb |
publicationDate |
2016-02-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
US-9255291-B2 |
titleOfInvention |
Oligonucleotide ligation methods for improving data quality and throughput using massively parallel sequencing |
abstract |
Described herein is a buffer concentration for highly efficient ligation of two oligonucleotides. The embodiments herein have led to the development of an optimized ligation step used in the sample preparation for sequencing reactions. Further, embodiments herein describe a high-throughput method for sequencing using barcodes or the purpose of multiplexing several samples simultaneously and novel methods for making targeted DNA libraries for re-sequencing on massively parallel next-generation sequencing platforms and for alternatives to gel-purification for recovering the desired templates from small RNA libraries for next generation sequencing. |
isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2023212560-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10072283-B2 |
priorityDate |
2010-05-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |