http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9206441-B2
Outgoing Links
Predicate | Object |
---|---|
assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_efbfdc7316d3b1b7ceaee5366a61b6e0 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_fe9efcec7d9ed77f844ba87d886fb92c |
classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B82Y5-00 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-87 |
classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/B82Y5-00 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-87 |
filingDate | 2012-12-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2015-12-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6f6661e688ce3fd96885a41921b7e5cd http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_56c0c1b6def28aed7781665ee7238483 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7be094e806f8dfcc801db9123df1ccc6 |
publicationDate | 2015-12-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | US-9206441-B2 |
titleOfInvention | Method for producing cell having nucleic acid introduced therein |
abstract | A method for producing a cell having nucleic acid introduced therein and having low cytotoxicity is useful in primary cells and slowly dividing cells, for which nucleic acid introduction is difficult with conventional techniques. A method for introducing nucleic acid into a cell includes the steps of mixing a nucleic acid with a temperature sensitive material at a temperature lower than the cloud point of the temperature sensitive material, the temperature sensitive material being formed by adding 2-amino-2-hydroxymethyl-1,3-propanediol to a temperature-sensitive polymer material having 2-N,N-dimethylaminoethyl methacrylate and/or a derivative thereof as the main polymer component, flow coating a culture vessel with the resulting mixed liquid, and culturing a cell suspension provided to the culture vessel at a temperature higher than the cloud point. |
priorityDate | 2011-12-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 472.