| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_07c9542186133097bd6bbb7d92393090
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_3bef9ecd84fbb8b06042ace974da4c51
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_13f6da7259a86918559628aadd719d79 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-86
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2770-36251
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2760-16051
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2795-14151
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2760-20211
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-70 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N7-00 |
| classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-86
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-00 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-70
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N7-00 |
| filingDate |
2006-11-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
2015-12-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f4e0c36a2da067ec071aa89dca9a1173
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0d4eadb7eb1e8a2416c897fa993edaba |
| publicationDate |
2015-12-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-9206396-B2 |
| titleOfInvention |
Methods and devices for quantitative viral assays |
| abstract |
A method for quantifying infectious particles of a virus in a sample comprises providing a layer of host cells of the virus, contacting the layer of host cells with a preparation of the sample, and culturing the cells under conditions wherein the cells are submerged in a thin layer of liquid culture medium, and wherein the virus infects host cells and releases its progeny from said infected host cells, imposing a flow of the liquid medium, wherein the spread of the viral progeny to uninfected host cells is enhanced, culturing the cells under conditions to allow further virus infection and viral gene expression, wherein infected host cells develop an observable indication of viral gene expression, and determining the number of infected host cells, whereby the number of infectious particles of the virus in the sample is quantified. The method may be used for measuring viral growth rate or for screening for antiviral compounds. Also provided are microfluidic devices suitable for the inventive method. |
| priorityDate |
2005-11-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |