| abstract |
The invention relates to a microfluidic system for controlling a card for the concentration of molecules capable of stimulating a target, for example formed by an assembly of living cells, characterized in that the system comprises a microfluidic device ( 1 ) comprising: n≧1 microfluidic channel(s) ( 4, 40 ), the or each channel being provided with at least one inlet orifice for at least one fluid and with at least one outlet orifice for this fluid; n≧2 openings ( 47, 470 ) formed in the microfluidic channel or distributed in the various microfluidic channels, said openings being arranged in one and the same plane so that they form a network having at least one dimension in this plane, the numbers n of microfluidic channel(s) and n of openings being linked by the relationship (I) with 1≦i≦n and n the number of openings for the channel c; at least one microporous membrane ( 5 ) covering the network of openings, the target being intended to be positioned on the side of the membrane which is opposite the microfluidic channel(s); one or more fluid feed means for feeding the or each microfluidic channel with fluid, at least one of these fluids comprising molecules for stimulating the target. |