| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_95d971fbbd33385a6098ee5555555ab1
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_55bb32af460428bf4fdd099027c20353 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2525-121
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2565-301
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2525-186
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2521-107 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-34
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-1252
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-68
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6844 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-34 |
| filingDate |
2013-06-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
2015-09-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d6478f527b42d7af89b4cb6e448a5ecc
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f6ae1655528bb0dde7fd64e40ca7a476 |
| publicationDate |
2015-09-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-9133491-B2 |
| titleOfInvention |
Pyrophosphorolysis-activated polymerization (PAP) using ribonucleic acid (RNA) template |
| abstract |
A new method of RNA-PAP was developed that can directly amplify RNA template without additional treatment. RNA-PAP brings in a new mechanism for amplification of RNA template in which RNA-dependent DNA pyrophosphorolysis and RNA-dependent DNA polymerization are serially coupled using 3′ blocked primers. Due to this serial coupling, RNA-PAP has high selectivity against mismatches on the RNA template, providing highly specific amplification of RNA template. In addition, mutant polymerases were genetically engineered for higher efficiency of RNA-dependent DNA pyrophosphorolysis and RNA-dependent DNA polymerization. |
| priorityDate |
2012-11-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |