Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_00d8a292f9cb8de0ddb88f6e78420e53 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_15322d49ffcddaf4f9ec2759d73d4731 |
classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01L9-52 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01L7-52 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01L2300-0867 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01L3-502784 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01L13-02 |
classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/B01L9-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/B01L7-00 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/B01L3-00 |
filingDate |
2008-10-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate |
2015-08-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5480f0aacce0884bdd6832e4be5450a5 |
publicationDate |
2015-08-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
US-9114397-B2 |
titleOfInvention |
Method of reducing cross-contamination in continuous amplification reactions in a channel |
abstract |
The present invention relates to a method for reducing cross-contamination in continuous amplification reactions in channels of microfluidic devices. More specifically, the present invention relates to the use of specific materials continuously flowing in the channels to reduce adsorption of MgCl 2 and the concomitant adsorption of nucleic acid template to the channel surface, thereby reducing cross-contamination. This reduction of cross-contamination improves the efficiency and reproducibility of the amplification reaction, e.g., PCR. |
isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111495450-A |
priorityDate |
2007-10-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |