| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_78941ed5a01bfbcd48376e1fdb485b19
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_602e60748d42fa06fd5942b203055ea1
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_44894ff0301f16d242d9f436df3c9de9 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y301-03048
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2334-20
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-42 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-573
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B82Y30-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B82Y5-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B82Y10-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07F9-65586
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07F9-65522
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-582 |
| classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-573
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-42 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-52
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-533
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07F9-655
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07F9-6558
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/B82Y30-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/B82Y5-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-58
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/B82Y10-00 |
| filingDate |
2006-07-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
2015-06-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_514431c6bef8d7f5ea9e2d351227e09b
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_925db88be50983eba1c940d4062fc80b |
| publicationDate |
2015-06-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-9045507-B2 |
| titleOfInvention |
Coumarin-based amino acids for use in enzyme activity and substrate specificity assays |
| abstract |
The design, synthesis, and utilization of a novel coumarin-based amino acid and its fluorinated derivatives for use in enzyme activity and substrate specificity assays are described. The synthesis is both facile and high-yielding and allows for the production of a highly fluorescent phosphorylated amino acid that can be incorporated into peptides using standard SPPS techniques. The resultant substrates are efficiently hydrolyzed by PTPs and exhibit a large increase in fluorescence upon hydrolysis. Fluorinated CAP derivatives have utility as fluorogenic enzyme substrates. CAP and its derivatives can be incorporated into combinatorial peptide libraries for assaying enzyme substrate specificities. |
| priorityDate |
2005-07-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |