| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_710cdaa7752ae059851d1dad2526b7f6
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_e2b0ca6ba79cc09ece94f276e2c349cf
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_d21cbff64022f95c237c0bd3ec8656ca
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_fe405aca762d774e0e31319702d3c09e |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2015-1486
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2015-1006
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2015-1081
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-70514 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N15-1031
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N15-1463
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-56972 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-569
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-53
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-555 |
| filingDate |
2013-03-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
2015-05-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2a5424eb6af78ba0c3b1fedd9e74a26f
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b3f6d5e041f40752d85f615c1e514dde
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_698939c5ad612dc12050ef2d77428c91 |
| publicationDate |
2015-05-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-9034589-B2 |
| titleOfInvention |
Magnetic separation of cells |
| abstract |
An apparatus, system, and method for magnetic separation of cells are disclosed. By combining inkjet printing technology and magnetic labeling of cells, accurate cell counts are obtained using an optical microscope. Mouse CD4+ lymphocytes are attached to micron sized magnetic beads and printed through a modified, commercial inkjet printer. The labeled cells are then attached to a glass slide covering a permanent magnet. Cell counts can be obtained by use of regular and inverted optical microscopes and imaging software. The magnetically-labeled beads are collected for evaluation on a modified polymer coupon that is placed in front of a permanent magnet and the unlabeled cells fall into an excess container. Flow cytometry results verify the presence of the CD4+ protein on the LBRM-33 lymphocytes membrane. Protein-specific attachment of magnetic microspheres to the lymphocytes is utilized for sorting CD4+ lymphocytes. |
| priorityDate |
2012-03-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |