http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9029076-B2
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_9782f345aeacefcfb39c08e3b7e6b001 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B82Y30-00 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01N1-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N1-30 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01N1-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N1-30 |
| filingDate | 2013-03-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2015-05-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_53ecb6450a2b26b7b76d4eba9dc39531 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_50852047e4a3568ff3286ae5f7feb714 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e057fa1560d96134122b8d71525de30c |
| publicationDate | 2015-05-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | US-9029076-B2 |
| titleOfInvention | Tissue preservation and fixation method |
| abstract | This invention relates, e.g., to a composition that, at room temperature, when contacted with a sample comprising phosphoproteins, can fix and stabilize cellular phosphoproteins, preserve cellular morphology, and allow the sample to be frozen to generate a cryostat frozen section suitable for molecular analysis. The composition comprises (1) a fixative that stabilizes the proteins in the sample and that has a sufficient water content for a stabilizer and/or a permeability enhancing agent to be soluble therein; (2) a stabilizer, comprising (a) a kinase inhibitor and (b) a phosphatase inhibitor and, optionally, (c) a protease (e.g., proteinase) inhibitor; (3) a permeability enhancing agent; and (4) lactic acid. Methods and kits are described for preserving phosphoproteins, using such a composition. Also described are endogenous surrogate markers for monitoring protein degradation, including the loss of posttranslational modifications (such as phosphorylation), e.g. following removal of a cell or tissue from a subject; and exogenous molecular sentinels (e.g. phosphoproteins attached to magnetic nanoparticles) that allow one to evaluate the processing history of a cellular or tissue population sample. |
| priorityDate | 2006-10-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 1482.