| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_cb004b277495e855e64bc6ac23f3e3ee
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_33b0aa448f6242902941f03f7c648a63
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_167db772dfe88f6b0cba534f8033c754
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_f373fea6cf39227df6256ade05fd39ac
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_e0ca52bf4c78a884f061198533f01e1c |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2523-32 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07H21-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6806
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-101
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1003 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H21-00 |
| filingDate |
2010-07-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
2015-05-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_aaa8109da39a17b003039ac24deec38c
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_32cb373eaa9bfc3e668738a7205dd3fe
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_dff896c72dc4d7bd7e3421ce8fbd457d
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fdf2e1642160abf23caddd9f2efc1756 |
| publicationDate |
2015-05-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-9024008-B2 |
| titleOfInvention |
Procedure for the specific isolation of total DNA content of bacterial germs and a kit for this purpose |
| abstract |
Procedure for the specific isolation of total DNA content of bacterial germs of different samples, in the course of which the cells are lysated, the DNA content of the lysate is bound selectively, it is washed and then the desalinated linear polymer nucleic acid is eluted from the binding surface in an aqueous solution. Before cell lysis the nonviable bacterial cells are separated from the viable cells on the basis of their different cell surface physical-chemical characteristics, the viable cells of the sample are kept and then lysated using a mechanical and/or enzymatic, favorably lysozyme enzymatic method. After this exclusively double-stranded DNA deriving from the lysate of viable cells is bound on a —SiO 2 —TiO 2- matrix containing chemically activated —OH and dodecylamine groups, and after washing it, the desalinated linear polymer nucleic acid is eluted in an aqueous solution. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11032709-B2 |
| priorityDate |
2010-07-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |