http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8999679-B2
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_ded8f1e029a01cb2e0959689cea4f446 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_102641a882d1cb61968495c694bd7476 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_04b9ffefde6d1d9ecee60d8bf8f28d5c http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_4ed3a253e268febd7efd8126bc95b888 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_2ed2f6b3a4e4af3d2136124706b35c24 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_a57e4edc308570c1c273cc4f66778508 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-64 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-66 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1093 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-64 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-66 |
| filingDate | 2009-12-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2015-04-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_55c11f1c2a17dbea30cda8f7d7e639fb http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c0423f177aa0ca8239b14a6bb81452cd http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d8c3da9af5c5b768d67a2d57af36fbf1 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_50a0e009447dce7b430d9236f20fada0 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a93e3187786751ef8f3147a26ec1785b |
| publicationDate | 2015-04-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | US-8999679-B2 |
| titleOfInvention | Method for assembly of polynucleic acid sequences |
| abstract | The present invention provides a method for the assembly of a polynucleic acid sequence from a plurality of nucleic acid sequences in which the polynucleic acid sequence is of a formula N n+1 , in which N represents a nucleic acid sequence and where n is 1 or greater than 1 and each N may be the same or a different nucleic acid sequence, in which the method comprises: (i) providing a first nucleic acid sequence N1 which has an oligonucleotide linker sequence L1 3 at the 3′-end of the nucleic acid sequence; (ii) providing a second nucleic acid sequence N2 which optionally has an oligonucleotide linker sequence L2 3′ at the 3′-end of the nucleic acid sequence and which has an oligonucleotide linker sequence L2 5′ at the 5′-end of the nucleic acid sequence, wherein the 5′-end linker sequence L2 5′ of nucleic acid sequence N2 is complementary to the 3′-end linker sequence L1 3′ of nucleic acid sequence N1; (iii) optionally providing one or more additional nucleic acid sequences N, wherein nucleic acid sequence N2 has an oligonucleotide linker sequence L2 3′ at the 3′-end of the nucleic acid sequence, and wherein said one or more additional nucleic acid sequences N comprises a terminal additional nucleic acid sequence NZ, and wherein each additional nucleic acid sequence N has an oligonucleotide linker sequence at its 3′-end, wherein said terminal additional nucleic acid sequence NZ optionally lacks an oligonucleotide linker sequence at its 3′-end and wherein each additional nucleic acid sequence N has an oligonucleotide linker sequence at its 5′-end, wherein for the first additional nucleic acid sequence N3 the 5′-end linker sequence L3 5′ is complementary to the 3′-end linker sequence L2 3′ of nucleic acid sequence N2 and for each second and subsequent additional nucleic acid sequence N the 5′-end linker sequence is complementary to the 3′-end linker sequence of the respective preceding additional nucleic acid sequence; and (iv) ligating said nucleic acid sequences to form said polynucleic acid sequence. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2014030766-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2018154384-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9777305-B2 |
| priorityDate | 2008-12-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 216.