| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_1d28879a9b44cb0ff0cbf6ac946c4b3a |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2527-107
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2525-301
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2549-101
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2533-101
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2525-207
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2537-143 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6809
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6844
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6848
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-686
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6853 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-34
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate |
2013-07-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
2015-02-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_671ce3862c33b9c78a92b9f1a868f2ba
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_08236a921a7a5b0fb3ff5be26b028751 |
| publicationDate |
2015-02-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-8962254-B2 |
| titleOfInvention |
Analyzing messenger RNA and micro RNA in the same reaction mixture |
| abstract |
The present teachings provide methods, compositions, and kits for performing primer extension reactions on at least two target polynucleotides in the same reaction mixture. In some embodiments, a reverse transcription reaction is performed on a first target polynucleotide with a hot start primer comprising a self-complementary stem and a loop, and extension products form at high temperatures but extension products form less so at low temperatures since the self-complementary stem of the hot start primer prevents hybridization of the target specific region to the target. However, non-hot start primers with free target specific regions can hybridize to their corresponding targets at the low temperature and extension can happen at the low temperature. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9422603-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2015232918-A1 |
| priorityDate |
2005-07-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |