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publicationDate 2015-02-03-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber US-8947510-B2
titleOfInvention Functional imaging of cells with optical projection tomography
abstract A method for 3D imaging of a biologic object ( 1 ) in an optical tomography system where a subcellular structure of a biological object ( 1 ) is labeled by introducing at least one nanoparticle-biomarker. The labeled biological object ( 1 ) is moved relatively to a microscope objective ( 62 ) to present varying angles of view and the labeled biological object ( 1 ) is illuminated with radiation having wavelengths between 150 nm and 900 nm. Radiation transmitted through the labeled biological object ( 1 ) and the microscope objective ( 62 ) within at least one wavelength bands is sensed with a color camera, or with a set of at least four monochrome cameras. A plurality of cross-sectional images of the biological object ( 1 ) from the sensed radiation is formed and reconstructed to make a 3D image of the labeled biological object ( 1 ).
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