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filingDate 2009-05-13-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2014-10-21-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_513b870a472e8d991a5e2ac087233055
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publicationDate 2014-10-21-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber US-8865399-B2
titleOfInvention Phage-mediated bioluminescent detection of Yersinia pestis
abstract The present disclosure relates to compositions, methods, systems and kits for the detection of microorganisms of the Yersinia species including Yersinia pestis . The disclosure relates to recombinant phage operable to infect a Yersinia microorganism, the phage comprising a detectable reporter. Detection systems of the disclosure may comprise a phage operable to infect a Yersinia microorganism, and may comprise a reporter nucleic acid expressible upon infection of a Yersinia microorganism by the phage. The system may be operable to detect the expression of the reporter. A detectable reporter may comprise any gene having bioluminescent, colorimetric and/or visual detectability. For example, a detectable reporter may comprise one or more luxAB genes detectable by emission, enhancement and/or change in spectrum of bioluminescent light. Live and infectious Yersinia microbes may be detected by the compositions, methods, systems and kits described herein.
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