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filingDate 2008-12-05-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2014-10-14-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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publicationDate 2014-10-14-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber US-8859260-B2
titleOfInvention Expression cassette for lactase dehydrogenase, transformed yeast and method of producing lactic acid
abstract A gene expressing cassette codes lactate dehydrogenase that is needed for prevention of deterioration in lactic acid yield and lactic acid production rate in continuous culture with simultaneous filtration of a yeast strain having a lactic acid-producing ability, which achieves high optical purity, high lactic acid yield and high lactic acid production rate simultaneously, a yeast strain having the cassette and a method of producing lactic acid by culturing the yeast strain. The lactate dehydrogenase-expressing cassette is a lactate dehydrogenase-expressing cassette, comprising a gene coding lactate dehydrogenase connected to a site downstream of a promoter, the promoter being a promoter of a gene showing a gene expression amount larger by 5 times or more than the average relative expression amount of all genes after 50 hours from start of culture in continuous culture with simultaneous filtration of a yeast strain having a lactic acid-producing ability.
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