| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_2cb64c11c314ad38135578a8403c18e4
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_4b0335964c50aa9a3a7f8e210f7fdcc4
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_cb51182003f5a2e4ce3342d14b423040
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_634c80f274ba7484fb1ab1a3e7562966
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_43876cc9584ee1351a9b346906539b17
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_0e2d252f10436ade45bc8035005e2ea3
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_3d6916f9125c572773e8e80f9e15d490
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_2658d45a8e9c71b9598e2bf83242bab3 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2523-313
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2523-101
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2527-125 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1058
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6869 |
| classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H21-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H21-04 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate |
2009-07-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
2014-09-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3e77c6d0d87985eeb157edd5dac81f24
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_cfa113c01a6809a3f8e0896b63a05f94
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_93a10d04eb937792d359ed4ffc2a7b09
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5b8b7bb941c20df9a71176ce257cc81b
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ceda6012abe65894a7955af4d99c8d03
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a690b2d18122f7b2cc6398edaa1a1a9a
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e0044fdc4ecbfb9a59569b8ff6355cfb |
| publicationDate |
2014-09-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-8841073-B2 |
| titleOfInvention |
Methods for identifying RNA segments bound by RNA-binding proteins or ribonucleoprotein complexes |
| abstract |
The present invention relates to a method for identifying a binding site on an RNA transcript, wherein the binding site binds to one or more binding moieties. The method includes, among other things, introducing a photoreactive nucleoside into living cells wherein the living cells incorporate the photoreactive nucleoside into RNA transcripts during transcription thereby producing modified RNA transcripts; reverse transcribing the RNA of isolated cross-linked segments thereby generating cDNA transcripts with one mutation wherein the photoreactive nucleoside is transcribed to a mismatched deoxynucleoside; amplifying the cDNA transcripts thereby generating amplicons; and analyzing the sequences of the amplicons aligned against the reference sequence so as to identify the binding site, wherein the sequences of each amplicon having a mutation resulting from the introduction of the photoreactive nucleoside is considered to be a valid amplicon comprising at least a portion of a binding site on the RNA transcript. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2015353991-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11242553-B2 |
| priorityDate |
2008-07-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |