| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_7f2dd29e06d8b6684c1b63d5fbfbced9
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_0ecd3a929c5407f762ab3261c2041808
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_2619e03ba0d307debb2d4a4208128dd3
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_4c03b04055a3d564a0a72ee9441ba019 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P21-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-0071 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-07
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-64
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-00 |
| filingDate |
2006-06-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
2014-08-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8673f09accbefa18973b779248dbdc43
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_480efc89ad4206074d1ca9a335f5c0d2
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f41d01f300098956d553d59030db0e1d |
| publicationDate |
2014-08-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-8815583-B2 |
| titleOfInvention |
Cell lines for expressing enzyme useful in the preparation of amidated products |
| abstract |
Cell lines are provided for expressing peptidylglycine alpha-amidating monooxygenase (PAM), or one of its two catalytic domains. High levels of enzyme expression are achieved while utilizing a non-animal source, low protein tissue culture medium. A robust two-step downstream purification results in high enzyme purity. Resulting PAM, or its PHM catalytic domain, is used to catalyze the enzymatic conversion of X-Gly to X-alpha-hydroxy-Gly or X—NH 2 (X being a peptide or any chemical compound having a carbonyl group to which a glycine group can be covalently attached). Methods of preparing preferred cell lines are also set forth. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9457086-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9526785-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9833411-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9744140-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9399017-B2 |
| priorityDate |
2005-06-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |