http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8765473-B2
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_de2d31add5b61794e5549b8cdfb5a0fd http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_9019c0ce5cb7b82c85e121f6cac86dce http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_3183ca77093d4ac9e1e54305fd2a72dc |
classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2533-00 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61L27-20 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61L27-24 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61L27-3804 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61L27-3895 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0697 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61L27-24 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61L27-20 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61L27-38 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-071 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-07 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-00 |
filingDate | 2006-11-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2014-07-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_967288064fb6da0f524840a725f5a12e |
publicationDate | 2014-07-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | US-8765473-B2 |
titleOfInvention | Method of preparing basement membrane, method of constructing basement membrane specimen, reconstituted artificial tissue using the basement membrane specimen and process for producing the same |
abstract | A basement membrane having a barrier function is formed by culturing alveolar epithelial cells or vascular endothelial cells on a fibrous collagen matrix coated with a polymer having a sugar chain that can localize a receptor that has an activity to accumulate a basement membrane component on the basal surface of the cells having an ability to form a basement membrane. A reconstructed artificial tissue is obtained by seeding and culturing desired homogeneous or heterogeneous cells on the basement membrane specimen constructed by the following process: (i) the cells having an ability to form a basement membrane adhered onto a support structure through a basement membrane are treated with a surface active agent; (ii) the lipid component of cells is lysed; (iii) the mixture of an alkaline solution and a protease inhibitor is used to lyse the protein remained on the surface of the basement membrane of the cells. |
priorityDate | 2001-09-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 95.