| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_a0ff60ddd065856e757f8b803c73292e
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_a7cfc6f92aeb40f2a6e49482bf1683a6
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_3fcb093a174d5182b5854b704cb09354
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_bd628145dd9a21e004d6de2ac2c40d88 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C11D3-382
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B09C1-08
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C11D3-0084
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C11D3-188
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C11D3-39 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-532
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-533
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-534 |
| filingDate |
2007-02-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
2014-05-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_341c129e008e4b5765ded9ef25b52e51
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d8d0ca9b1e97c8ce12711a8670b29029
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4d358e097b4f9a05b7da367a5428a4eb |
| publicationDate |
2014-05-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-8735178-B2 |
| titleOfInvention |
Withanolides, probes and binding targets and methods of use thereof |
| abstract |
Novel withanolide chemical genetic probes identify the in vivo binding target of withaferin A, which is the intermediate filament type III protein vimentin. In addition, a withanolide-based small molecule screening method screens drug candidates that target intermediate filament type III proteins. The method includes introducing a tagged linker covalently bonded to the withanolide molecule to form a withanolide probe. Better or alternative small molecule compounds as potential drug candidates can be generated based on their likely affinity for the determined binding site in vimentin. The affinity labeled withanolide can also be used to find intermediate filament-associated proteins using chemical proteomics by extracting proteins from cells that were exposed to withanolide-biotin analog. The withanolide probes can be used to monitor expression of vimentin, in tumor samples or other diseased tissues. Withaferin analogs can be used as a treatment for diverse vimentin-associated disorders, such as cancers, angiofibrotic diseases, and chronic inflammation. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10136652-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9624437-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2018045738-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10690683-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9526692-B2 |
| priorityDate |
2006-03-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |