| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_a93917981305270638f59d48187ce53a
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_fb0bc7c632a0fa7848b9e00c38e91cf5
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_b4d2ea08e27e0c2a4f7e86890fe7af3f
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_517f36afd6943308aad8079b41499432
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_5189084524a8dc1554d21d5f4c8d936a
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_a2080e3d36502de777c9cd71ff1c152e |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01L2400-0406
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01L2300-1827
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2531-113
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01L2200-16
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01L2200-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01L7-52 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-686
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6851
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01L3-5027
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6837 |
| classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/B01L7-00 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-34
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/B01L3-00 |
| filingDate |
2007-10-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
2014-04-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d86cee76d57d2697b12c0cb3b7a3f9fb
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5d35bb781c408e39b9ba14be0882edc4
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5e93161b1c605fa4a33fcbef3e5a0d70
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d2f89677ab99d6849d12e692b5172b47
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7baa47ed849480dd8d600df6889e7597 |
| publicationDate |
2014-04-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-8697362-B2 |
| titleOfInvention |
Methods of detecting low copy nucleic acids |
| abstract |
Methods are provided for detecting low copy nucleic acids of interest in a sample. In one method, a sample comprising a nucleic acid of interest is aliquotted into a plurality of reaction mixtures, at least two of which are single-copy reaction mixtures. The reaction mixtures are subjected to one or more amplification reactions while flowing through a channel of a microfluidic device. At least one of the reaction mixtures is formulated in an aqueous phase of an emulsion comprising aqueous droplets suspended in an immiscible liquid. The nucleic acid of interest is present as a single copy in at least one aqueous droplet of the aqueous phase prior to performing the amplification reaction(s). Amplification is performed on the reaction mixture when it is formulated in the emulsion. The nucleic acid is continuously flowed during a plurality of steps of the method. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10946384-B2 |
| priorityDate |
2002-12-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |