| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_8e31a5aff1483bd050c82a95e31bbcb0
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_9f3daa1bee8fc89ced9ba51855efc237
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_b1ab7461617f346aca038b9747c75ffb
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_2a033e54bb1acd5fd5b03db32cea51d9
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_be2c55a9de09e08e3404c0a5a1ae4dc7 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-18 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-1136 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A23J1-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K16-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K17-00 |
| filingDate |
2011-06-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
2014-01-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0ee972670db6c23f2e713a25b77a9f7a
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a4680e8eecf52562f5bb3b787d4e922a
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1673f51c4d3a8620c7ca624557e6cba9
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_07628f327fefe988272493ef080ab605 |
| publicationDate |
2014-01-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-8624005-B2 |
| titleOfInvention |
Method of protein refolding with ion exchange resins and the application of the same |
| abstract |
A method for protein refolding with an ion exchange resin. The method includes (a) choosing an ion exchange resin that having charged groups with the same sign as a net charge of a denatured protein to be refolded; (b) removing heterogeneous ions from the ion exchange resin by washing the ion exchange resin sequentially with saline solution and deionized water, to prepare the ion exchange resin; (c) mixing the ion exchange resin with a refolding buffer thoroughly, then adding the denatured protein to the refolding buffer and allowing the denatured protein to refold; and then (d) collecting the supernatant by centrifugation or settlement, to obtain a solution containing the refolded protein. |
| priorityDate |
2010-10-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |