| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_6102ae22a04c36dc7eef027ea8b11da4
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_1f1f35633312a8d06bd38f8e9a88073a
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_458ac9bf91b324d8b4efdb4673cc9026
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_8791d2e4a0fe1814428eda50c4e0590f |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-112
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-16
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-156 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6886
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6827
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6841
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6809 |
| classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H21-04 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate |
2012-02-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
2013-10-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b1af95bfc4cc469141e78bdd46a30607
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_cd95ffbed7d16659364596af8465a6a9
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e1d106aa4707f2b6dd6efa7b1fe8923a |
| publicationDate |
2013-10-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
US-8551708-B2 |
| titleOfInvention |
Methods for localized in situ detection of mRNA |
| abstract |
The present invention relates to the detection of RNA in a sample of cells. More particularly, the present invention relates to the localized detection of RNA in situ. The method relies on the conversion of RNA to complementary DNA prior to the targeting of the cDNA with a padlock probe(s). The hybridization of the padlock probe(s) relies on the nucleotide sequence of the cDNA which is derived from the corresponding nucleotide sequence of the target RNA. Rolling circle amplification of the subsequently circularized padlock probe produces a rolling circle product which may be detected. Advantageously, this allows the RNA to be detected in situ. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9376711-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2015011416-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11629342-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2018226930-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2014302496-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109112061-A |
| priorityDate |
2011-02-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |