http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8541206-B2
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_ca32fd2a44fd326ffd027a75a57bd13f http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_5777886c433148c0d6f3d41dbce5ae99 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_3f769b6b3406d6c3d799a58d97d443f2 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6827 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-34 |
| filingDate | 2006-12-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2013-09-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e48fd85a02b06f8ad3f7c738917a10ec http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5ab99cc2c17252394bd22be08659db5b |
| publicationDate | 2013-09-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | US-8541206-B2 |
| titleOfInvention | Differential amplification of mutant nucleic acids by PCR in a mixture of nucleic acids |
| abstract | A method for enriching a mutant nucleic acid in a mixture of nucleic acids, wherein the method comprises: (a) providing a nucleic acid mixture comprising a parental nucleic acid and a mutant nucleic acid of the parental nucleic acid; and (b) amplifying the nucleic acids in the nucleic acid mixture by polymerase chain reaction (PCR); wherein the mutant nucleic acid is a G→A mutant of the parental nucleic acid, which pairs with a fully complementary nucleic acid sequence to form an AT-rich nucleic acid variant of the parental nucleic acid; and wherein the AT-rich nucleic acid variant is denatured and selectively amplified by carrying out PCR using a denaturation temperature 1-3° C. lower than the lowest denaturation temperature (T p ) that allows amplification of the parental nucleic acid to thereby enrich the mutant nucleic acid in the nucleic acid mixture. |
| priorityDate | 2005-12-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 58.