http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8507295-B2
Outgoing Links
Predicate | Object |
---|---|
assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_9e0a4426e980ca1dc3bc34f2f1c4eb34 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-54306 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-54388 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-558 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-558 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-543 |
filingDate | 2008-11-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2013-08-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c795e467cb7b9dfa61c93bac277ed6cf |
publicationDate | 2013-08-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | US-8507295-B2 |
titleOfInvention | Methods of quantification for lateral flow devices |
abstract | The invention concerns methods of quantification of an analyte, A, in a test sample by means of a single immunochromatographic device, such as a lateral flow device (LFD). One method comprises the steps of: a) mixing a determined amount of said test sample with a determined amount of a quantification agent, QA 1 , said QA 1 being capable of binding specifically and simultaneously both to an immobilized binding partner and to A, thus obtaining a mixture wherein the amount of A in said test sample is reflected by the ratio of the concentration of A-QA 1 complex formed to the concentration of free QA 1 in said mixture; b) applying a determined volume of said mixture to said device equipped with a test band in which said binding partner of the QA 1 is immobilized, so that A-QA 1 complex and free QA 1 are immobilized at said test band in a ratio that relates to their concentration ratio in the mixture applied, said volume of mixture being determined to provide a total amount of A-QA 1 complex and free QA 1 capable of sufficiently saturating the immobilized binding partner in said test band; c) measuring the amount of either said A-QA 1 complex or said free QA 1 immobilized at the test band by means of an appropriately chosen detection procedure; d) comparing the result obtained from the test sample with the results obtained from calibration samples containing known or allocated amounts of A and thus determining the amount of A in said test sample. By including the two procedural stages a) and b) this greatly diminish or eliminate the sources of run-to-run variation. |
isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11287424-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9885712-B2 |
priorityDate | 2007-11-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 34.