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filingDate 2008-03-07-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2013-07-16-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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publicationDate 2013-07-16-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber US-8486139-B2
titleOfInvention Method of preparing decellularized soft tissue, graft and culture material
abstract It is intended to provide a method of preparing a decellularized soft tissue whereby swelling after an ultrahigh pressure treatment can be inhibited, etc. The above-described method of preparing a decellularized soft tissue comprises the application step wherein an ultrahigh hydrostatic pressure is applied to an isolated soft tissue in a medium to thereby disrupt cells in the soft tissue, and the removal step wherein the disrupted cells are removed. As the medium, use is made of an aqueous solution containing a water-soluble polysaccharide such as dextran.
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